Characterization of an ultra-high performance immunosensor modified with sol-gel nanogold particles and its applications

Our research group is developing a series of biosensors that are capable of measuring metabolites and chemical species at extremely low levels for various biomedical and environmental applications, such as DNAs and biomarkers for cancer detections as well as pollutants for environmental monitoring. As a demonstrative model in this study, we are reporting an immunosensor fabricated in our laboratory for the detection of human immunoglobulin G (HIgG) which is the smallest but the most common anti-infection antibody that makes up of majority (75-80%) of all antibodies in our body. HIgG can bind with many pathogens and neutralizes invading toxins. The level of IgG concentration within an individual is a good indicator of the person’s immune status. The immunosensor consists of an anchoring electrode that is modified with a layer of biocomposite; the biocomposite comprises coatings of polymer (cysteamine/melamine), nano Au particles, and anti-HIgG. In this report, the detection range of this immunosensor from ...