A simple pre-column derivatization method for the determination of mancozeb technical (fungicide) by reverse phase HPLC-UV

A simple and fast pre-column derivatization high performance liquid chromatographic method for the technical analysis of mancozeb was developed and validated in the present study. The mobile phase consists of a mixture of acetonitrile and 0.1% (v/v) formic acid in the proportion 60 : 40. This was found to give a sharp peak of the S-ethyl derivative of mancozeb (S-ethyl ethylene bis-dithiocarbamate) at a retention time of 9.12 minutes. HPLC analysis of mancozeb was carried out at a wavelength of 272 nm with a flow rate of 1.0 mL per minute. The linear regression analysis data for the calibration curve showed a good linear relationship with a regression coefficient of 0.999 in the concentration range of 8.2 mg L−1 to 32.3 mg L−1 for the derivative of mancozeb technical. The method was validated for specificity, linearity, precision and accuracy.