RAMAN SPECTROSCOPY INVESTIGATIONS OF THE CD1 NU/NU MOUSE SKIN PRE-CARCINOMA DAMAGES

Cancer is one of the most studied pathology and early detection could be helpful for treatment or other types of interventions that may stop the fatal evolution. The preclinical studies can be applied on experimental models, especially on rodents. Cancer can be induced experimentally most often by the application of chemical carcinogens, tumor cells inoculation or xenografts. FT-Raman spectroscopy in conjunction with the surface enhanced Raman scattering (SERS) has been applied for preliminary investigation on skin tissue from mice models in order to evaluate the possibility to record analytical spectral fingerprint in tissue diagnostic. FT-SERS spectra from the UV and chemically damaged tissues showed dominant characteristic bands from collagen and nucleic acids, whereas the bands attributed to proteins are observed with less extent. Rezumat Cancerul este una din patologiile cele mai studiate, iar detectarea timpurie ar putea fi de folos in aplicarea unui tratament, sau a altor tipuri de intervenţii ce pot opri evoluţia fatală. Studiile preclinice se pot aplica pe modele experimentale, mai ales pe rozătoare. Inducerea cancerului se poate realiza cel mai adesea prin aplicarea carcinogenilor chimici, inoculare de celule tumorale sau xenogrefe. Spectroscopia vibraţională Raman cu transformata Fourier (FT-Raman) combinată cu tehnica de amplificare a semnalului, SERS (surface enhanced Raman scattering), s-a aplicat pentru investigaţii preliminare ale ţesutului cutanat prelevat de la modele de soarece, pentru a evalua posibilitatea inregistrării amprentei spectrale analitice in diagnosticarea tisulară. Spectrele FT-SERS inregistrate in cazul ţesuturilor afectate de expunerea la UV sau chimic, au prezentat benzi dominante caracteristice pentru colagen si acizii nucleici, in timp ce benzile atribuite proteinelor au fost mai slab reprezentate.