Thedetectionofgastriccancercellsinintraoperativeperitoneal lavage using the reverse transcriptioneloop-mediated isothermal amplification method

Introduction: To detect a small number of malignant cells, we used a highly sensitive detection system that measures the expression levels of cytokeratin (CK) 19 messenger RNA by reverse transcriptioneloop-mediated isothermal amplification (RTeLAMP). Materials and methods: We evaluated the clinical relevance of our novel diagnostic method with an RTeLAMP assay using CK19 as a target gene for the detection of free cancer cells in peritoneal lavage and assessed the clinical significance of the molecular diagnosis by survival analysis and frequency of recurrence, with a median follow-up period of 39 mo. We observed 52 patients with gastric cancer who underwent gastrectomy, bypass