Chemical Composition, Antibacterial and Antifungal Potential of an Extract From the Leaves of Guapira Graciliflora Mart. Against Oral Microorganisms of Dental Interest

Objective:  To perform an  in vitro  analysis of antibacterial and antifungal potential of an alcoholic extract from the leaves of  Guapira Graciliflora  Mart. against oral microorganisms and determine its chemical composition.  Material and Methods: A hydroalcoholic extract of the leaves form  G. graciliflora  was obtained through maceration, vacuum concentration and freeze-drying. Antibacterial and antifungal activities were evaluated against  Streptococcus mutans ,  Streptococcus salivarius ,  Streptococcus oralis ,  Streptococcus parasanguinis ,  Streptococcus mitis  and strains of  Candida albicans  using broth microdilution method. Phytochemical analysis determined the total phenolic compounds, protein concentration and total of sugars present in the extract .  Results: G. Graciliflora  demonstrated antifungal activity against the LM 11 and LM 410 clinical isolates of  C. albicans  (MIC 0.5 mg/mL and 2 mg/mL, respectively). The other microorganisms tested were resistant to the extract. The phytochemical analysis revealed 3% proteins, 13% total sugars and 17% phenolic compounds .  Conclusion: G. Graciliflora  has antifungal activity against clinical strains of  C. albicans  and exhibits proteins, sugars and phenolic compounds in its chemical composition.