Development and Validation of a RP-HPLC method for Analysis of Cidofovir in Medicinal Form

Objective: A novel RP-HPLC method has been developed for analysis of cidofovir in its available medicinal form. Method: Stationary phase was a sophisticated C-18 RP Column (250mm × 4.6mm). Mobile phase consisted of Ethylnitrile and 0.005M citric acid buffer (pH adjusted to 5.5) in 60:40 V/V ratio. Mobile phase flow rate was 1.0 ml per minute and Isocratic. UV detection wavelength was 260nm. Validation was carried according to ICH guidelines. Findings: The Retention time of both AP1 and the medicine was 3.88 minutes. Linearity was satisfied over the concentration range of 2 ppm to 10 ppm and r² value was 0.999. LOD and LOQ values were 0.067 ppm and 0.205 ppm respectively. Improvement: The Reported method was novel and all validation parameters met the criteria. Method can be used in quality control analysis.