Evaluating the anti-leech ( Limnatis nilotica ) activity of methanolic extract of Allium sativum L. compared with levamisole and metronidazole

This study was carried out to detect the effects of methanolic extracts of Allium sativum L. on Limnatis nilotica. In this experimental study in September 2010, a number of leeches (70 in total) from the southern area of Ilam province were prepared, the effects of the methanolic extract of A. sativum L. with levamisole and metronidazole as the control drugs were compared, and distilled water was evaluated as the placebo group which investigated L. nilotica using anti-leech assay. The average time of paralysis and death of Limnatis nilotica for Metronidazole, methanol extract of Allium sativum L. and Levamisole was 718.77 ± 66.3 min, 5.11 ± 1.76 min and 144.55 ± 57.217 min, respectively. Distilled water and garlic tablets at a dose of 400 mg were determined as the inert group. In this research, the attraction time of death of leeches among different treatments is significant. In this study, it was determined that levamisole, with an intensity of 4+, and methanolic extracts of A. sativum L., with an intensity of 2+, have a good anti-leech effect and can be shown to be effective in cases of leech biting, while metronidazole and distilled water were negative.