Livestock embryo vitrifying freeze process on metal surface

The present invention relates to "animal embryos metal surface vitrification (Metal Surface Vitrification, MSV)", belongs to the field of biotechnology embryo. Livestock embryos vitrification metal surface, comprising a cryoprotectant liquid displacement embryonic intracellular freezing step and flash frozen embryos droplet, wherein: the upper surface is quickly frozen embryos frozen in liquid drops of a metal block, the metal block suspended or immersed in liquid nitrogen, and liquid nitrogen on the surface exposed to the surface. The present invention can greatly improve the cooling rate of the embryo, the embryo to avoid liquid nitrogen contamination of impurities; cytoskeleton added simultaneously in a freeze-protecting agent solution - cells, cytochalasin B, antifreeze ability to enhance the embryo, the effect of improving vitrification, When used for cattle embryo freezing, so that the recovery rate after IVF blastocyst freezing and thawing than 96%, 80-85% hatchability.