ÜÇ FARKLI SİLİKON ELASTOMERİNDE CANDİDA ALBİCANS TUTUNUMUNUN (ADHERENCE) KARŞILAŞTIRILMASI-COMPARISON OF ADHERENCE OF CANDİDA ALBİCANS ON THREE SILICONE ELASTOMERS

OZET Candida albicans, protez materyallerine tutumunu ile olusturabilecegi lokal ve sistemik enfeksiyonlar nedeniyle materyallerin kullaniminda bir dezavantaj olarak karsimiza cikmaktadir. Bu nedenle cene-yuz protezlerinde siklikla kullanilan bir materyal olan silikonun Candida albicans tutunumu acisindan degerlendirilmesi ve farkli silikon elastomerlerinin bu acidan karsilastirilmasi onem tasimaktadir. Bu calismada uc farkli silikon elastomeri (VST-50, MDX4-4210 ve A2000-1) (Factor II Inc., Lakeside, Arizona) yuzeyinde mantar tutunumu arastirildi. Her bir elastomerden 0,5cm x 1cm boyutlarinda onar ornek hazirlandi. Ornekler sterilize edildikten sonra Candida albicans ekilmis 30 ml'lik Sabouraud besiyerine yerlestirildi. Enkube edilen orneklerin yuzeylerindeki maya kolonizasyonunun incelenmesi icin spektrofotometrede bulaniklik olcumu yapildi. Ayni islemler bir kez de uzerleri tukuruk ile kaplanmis orneklerde uygulandi. Sonuclar istatistiksel olarak elastomeri erin kendi iclerinde yapilan olcumlerde "Paired T-Test", birbirleriyle kiyaslamada ise "Tek yonlu varyans analizi" yontemleri kullanilarak degerlendirildi. Tukurukle kaplama oncesinde materyaller arasi C.albicans tutunumu acisindan istatistiksel olarak anlamli bir fark olmadigi (p>0.05) goruldu. Tukurukle kaplandiktan soma A2000-1 silikon elastomerinde, MDX4-4210 ve VST-50 elastomerlerine gore anlamli derecede daha az tutumun gozlendigi sonucuna varildi. Uc elastomerin her biri icin ayri ayri yapilan tukurukle kaplama oncesi ve sonrasi degerlendirmelerinin sonuclari incelendiginde ise A2000-1 tipi silikon materyalinde tukurukle kaplama oncesi ve sonrasi mantar tutunumu acisindan istatistiksel olarak anlamli bir fark gozlenmezken (p>0,05); MDX4-4210 ve VST-50 materyallerinde anlamli olcude artan tutunum gozlendi. Anahtar kelimeler: Candida albicans, silikon elastomeri SUMMARY The purpose of this study was to evaluate the adherence of Candida albicans to the maxillofacial prosthesis commonly employed in material-silicone and to compare silicone elastomers. In this study fungal adherence to three silicone elastomers (VST-50, MDX4-4210 and A2000-1) (Factor II Inc., Lakeside, Arizona) was investigated. Ten specimens of each elastomer were prepared (1cm x 0,5 cm). The sterilized specimens were placed in Sabouraud broth (30 ml) contaminated with Candida albicans. In order to investigate the fungal colonization on surfaces of incubated specimens, turbidity was measured in spectrophotometer. The same procedures were carried out with saliva coated specimens. Results were analyzed with Paired T-Test and with one-way ANOVA. There was no significant difference among all uncoated materials in C. albicans adhesion (p>0.05); but there was a statistically significant difference among the coated materials. Saliva coated A2000-1 silicone elastomer showed a significantly less adhesion then the coated MDX4-4210 and VST-50 elastomers, With analysis of fungal adherence in coated and uncoated specimens for each elastomer, no significant difference was observed between coated and uncoated A2000-1 elastomers, but significantly more adhesion was observed in coated specimens for both MDX4-4210 and VST-50 elastomers. Keywords: Candida albicans, silicone elastomers