Diversity of Microflora Surviving Decontamination Treatments of Pasteurized Meat Carcasses Assessed Using Cultivation-based and Nested PCR-DGGE Fingerprinting Methods Poblational Study
2021
The diversity of microflora was determined to assess the effectiveness of decontamination treatments on pasteurized
meat carcasses at a large meat packing plant in Canada. The main objectives were to characterize the bacterial
diversity surviving in the heat-treated and DNase-I-treated samples over the untreated samples of pasteurized meat
carcasses. Cultivation based-methods were combined with nested PCR-DGGE fingerprinting methods to quantify
low numbers of bacterial survival in each sample. Using the DGGE reference marker, seven genera (Pseudomonas,
Staphylococcus, Propionibacterium, Chryseobacterium, Flavobacterium, Ralstonia, Paenibacillus) were detected by both DGGE
and cultivation-based method. Three species (Streptococcus salivarius, Micrococcus luteus and Leuconostoc mesenteroides)
were exclusively found in pure cultures with cultivation-based method. Over fifteen genera were found by PCR-
DGGE using both the DGGE marker strains and bands in real samples, indicating the highest diversity determined
by this technique. By contrast, the highest quantity of species was detected by cultivation-based method (29 and
88 in heat 34 treated and untreated samples, respectively). Five E. coli isolates in the family of Enterobacteriaceae were detected in untreated samples with plating methods demonstrating the usefulness of processing meat samples
with decontamination and heat treatments. However, most of the unidentifiable species or genus by cultivation-
based were almost detected by PCR-DGGE, confirming the effectiveness of combining both culture-dependent and
independent methods to completely profile bacteria in food samples.
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
0
References
0
Citations
NaN
KQI