Genes and transcription factors, including nuclear receptors: Methods of studying their interactions

Abbreviations: bp base pair; CAT chloramphenicol transferase; cDNA complementary DNA; mRNA messenger RNA; EMSA electrophoretic motility shift assay; ER estrogen receptor; ERE estrogen-responsive element; ERR estrogenrelated (orphan) receptor; GRIP-1 glucocorticoid receptor–interacting protein; GST glutathione S-transferase; GR glucocorticoid receptor; GTH glutathione; HSP heat-shock protein; IPTG isopropylthio-D-galactopyranoside; LBD ligand-binding domain; Luc luciferase; NR nuclear receptor; PAGE polyacrylamide gel electrophoresis; PR progesterone receptor; RAR retinoic acid receptor; RE response element; SDS sodium dodecyl sulfate; SFRE SF1-responsive element; SPR surface plasmon resonance; SRC steroid receptor coactivator; TATA box thymineadenine-thymine-adenine sequence; TF transcription factor; TIC transcription-initiation complex; TIF transcription intermediary factor; TBBP TATA box–binding protein; TR thyroid hormone receptor; TRE thyroid hormone response element; TRAP TR-associated protein