Use of chemosynthetic crrna and modified crrna in crispr/cpf1 gene editing systems

The present invention is based on the use of the CRISPR/Cpf1 system in gene editing, wherein a1) or a2) or a3) is included in the CRISPR/Cpf1 system: a1) a chemosynthetic crRNA; a2) a chemosynthetic and modified crRNA; and a3) a crRNA expressing vector. The modification method is modification of a deoxyribonucleic acid. The deoxyribonucleic acid is a thymidine deoxynucleotide. Experiments show that the chemosynthetic crRNA and the chemosynthetic and modified crRNA for the CRISPR/Cpf1 system both result in mutations in the hAAVS1 gene and THUMPD3-AS1 gene, that is, both have a certain gene editing ability. The crRNA expressed by a vector, the chemosynthetic crRNA and the chemosynthetic and modified crRNA for the CRISPR/Cpf1 system have an important application value in gene editing.