Experiments to isolate cytopathic strains of bovine rotaviruses

: Experiments were carried out to isolate cytopathic strains of bovine rotaviruses. Material from a total of 129 positive fecal samples, taken from calves affected with rotavirus gastroenteritis, was used to infect cell cultures. In the roller culturing of 19 of the positive samples in suspension of the MA-104 cell line in the presence of trypsin two cytopathic strains were isolated. The cell line used was more sensitive than the cells of calf kidney.