Confrontation of the “Dual Tracer” Indirect Method With Direct Ileal Sampling for Indispensable Amino Acid Digestibility of Sunflower Isolate in Humans

Objectives The direct assessment of ileal samples of amino acid (AA) digestibility is invasive in humans. A less invasive but indirect method, namely « dual tracer » was recently developed. It relies on the plasma isotopic enrichment ratio of two labeled protein, a reference protein or alternately AAs labeled with 13C and the test protein labeled with 15N. This recent method has not yet been challenged against direct measurement of ileal digestibility.