Recombinant human papilloma virus subtype 33 expression

The present invention discloses a codon-optimized human papilloma virus major capsid protein L1 encoding gene, and after the gene is transformed into yeast cells, human papilloma virus major capsid protein L1 can be efficiently expressed. The invention also discloses an immunogenic macromole, which is mainly produced by expression of the codon-optimized human papilloma virus major capsid protein L1 encoding gen in the yeast cells. The invention also discloses application and a composition of the immunogenic macromolecule.