Establishment of a New Herbicides Screening Method Using Photoautotrophic Cultured Cell II. The Responses of Chlorophyllous Cells to Paraquat and Diuron in Tabacco

This study was conducted to determine the response of newly developed chlorophyllous cells against photosynthesis inhibitory herbicides in LS medium. Inhibition of the growth of the selected chlorophyllous cells in the LS medium containing sucrose 1%, NAA M and BA M under light condition increased as the concentrations of paraquat increased from M to M. The calli died in M paraquat treatment and the inhibition of calli growth was greater when was supplied. In the treatment of herbicide diuron, the inhibition of calli growth also increased as the concentrations of diuron increased from M to M and more inhibition was observed at 1% sucrose than 2% sucrose.