Isolation, Characterization and Elucidation of Invigorative Potential of Flavonoid From Stem-Bark of Prosopis cineraria on LPS-induced Oxidative Stress and Inflammatory Cascade in Swiss Albino Male Mice

The present research aimed to elucidate the structure and characterize the isolated compound from stem-bark of Prosopis cineraria and unravel its potential against LPS-induced toxicity in mouse model. The spectral techniques were done for characterization and structure elucidation of the isolated compound (HPLC, NMR, FT-IR, LC-MS. The experimental mice were intoxicated (intra-peritoneal) with LPS (2 mg/kg body weight) and further treated with isolated compound from Prosopis cineraria (15 mg/kg body weight). Dexamethasone was used as a standard (10 mg/kg body weight). The oxidative stress parameters (LPO, CAT, SOD, GSH, GST and GPx) and biochemical activities (AST, ALT, ACP and ALP) were studied. The levels of pro-inflammatory cytokines (TNF-α; Prostaglandins E2; IL-6; NF-κBp65; IFN-γ and IL-10) were determined in liver homogenate. Nitric Oxide (NO) produced due to LPS-intoxication was determined by using Griess reagent. The results of the spectral analysis were used to elucidate the structure of the isolated flavonoid. The isolated flavonoid suppressed the over-expression and altered levels of oxidative parameters and cytokines due to LPS intoxication and restored the levels of TNF-α, NF-κB, NO, IL-6, IFN- , Prostaglandin E2 and IL-10. The research investigation unfolded the alleviating potential of the isolated compound against LPS-induced adverse effects by modulating the expression of cytokines and combating oxidative stress.