Device and method for operating multiple adherent cells on same substrate

The invention relates to a device and a method for adhering multiple cells to the same substrate and operating the cells, which divide a gold surface of the substrate into a modifiable region and an unmodifiable region by using a microflow pipeline of PDMS. The method comprises the following steps: introducing different molecular modifications to different pipeline regions to ensure that the substrate is divided into a cell adhesive region and a cell nonadhesive region; inoculating different cells in different adhesive regions, taking away the PDMS, and adhering multiple cells to the surface in sequence; electrochemically desorbing to destroy combination of molecules and gold in the nonadhesive region to ensure that all kinds of cells can move freely; and selectively modifying two sides of one or more cells as the nonadhesive region, separating other cells by using PBS, removing PDMS, and fixing the selected cells to lead other cells to move freely. The method and the device have simple and easy operation, can control fixation, complete motion or partial motion of different cells to achieve the aim of operating multiple cells at will, and can be used for studying interaction amongmultiple cells and medicinal screening.