Effect of α1, 2-fucosyltransferase gene transfection on tumorigenicity and angiogenic growth factor expression in ovarian carcinoma-derived RMG-I cells of nude mice.

Objective To compare the changes in tumorigenicity and expressions of vascular endothelial growth factor(VEGF),transforming growth factor-β1(TGF-β1),and basic fibroblast growth factor(b-FGF)in ovarian carcinoma-derived RMG-I cells of nude mice before and after transfection of α1,2-fucosyltransferase(α1,2-FT)gene.Methods Previously,we transfected the ovarian cancer cell line RMG-I with α1,2-FT gene using gene transfection technology and established cell line RMG-I-H with high expressions of α1,2-FT and Lewis y antigen.In this study,nude mice were subcutaneously injected with RMG-I-H and RMG-I cells.After 5 weeks,the mice were killed,and the weight of formed tumors was measured.Tumorigenicity was observed,and the expressions of VEGF,TGF-β1,and b-FGF in tumor tissue were detected by immunohistochemistry.Results Subcutaneous tumor was formed in nude mice in both transfected and non-transfected groups.The time of tumor formation in transfected group was earlier than that in non-transfected group(5.2±0.8 vs.8.8±1.3 d),and the tumor weight and volume in transfected group were significantly increased compared with non-transfected group(P 0.05).The expressions of VEGF,TGF-β1,and b-FGF in transplanted tumor tissue was significantly higher in transfected group than in non-transfected group(P 0.05).Conclusion α1,2-FT gene transfection can enhance the tumorigenicity of RMG-I cells in vivo and up-regulate the expressions of VEGF,TGF-β1,and b-FGF in transplanted tumor tissue.These results suggest that α1,2-FT and Lewis y antigen may be involved in angiogenesis,and the high expressions of α1,2-FT and Lewis y antigen may play important roles in the promotion of angiogenesis.