Stoichiometric analysis by chromatographie techniques I. Analysis of some antioxidants and penicillins by HPLC methods

Abstract An HPLC method has been utilized to follow a titration by monitoring either the reactant(s), product(s) or both. Such techniques produce linear titration curve(s) and the end point is determined from extrapolation to zero reactant concentration and/or maximum product concentration. This procedure has been successfully applied to the Stoichiometric analysis of several antioxidants and antioxidant mixtures. This technique has also been used to obtain useful information about the stoichiometry, kinetics and mechanism of the hydroxylamine reactions of ampicillin, cyclacillin and 6-aminopenicillanic acid. This method is superior in specificity, sensitivity and scope over conventional titration Stoichiometric methods. This study shows that the HPLC method is a powerful tool for conducting and exploring Stoichiometric analysis, the basis of analytical chemistry.