Relationship of GABA_A Receptor with Sevoflurane-induced Spontaneous Motor Activity Inhibition and Amnesia in Juvenile Mice

OBJECTIVE:To investigate relationship of γ-aminobutyric acid type A(GABA A)receptor with sevoflurane(S)-induced spontaneous motor activity inhibition and amnesia in juvenile mice.METHODS:Juvenile mice were randomly divided into 3 groups(n=80)and included in spontaneous motor activity test,step-down test and step-through test.Each group was subdivided into blank control group(NS 10 ml/kg),S group(3.0 ml/kg),special GABA A receptor blocker Securinine(Se)low-dose,medium-dose and high-dose groups(2,4,8 mg/kg),drug combination low-dose,medium-dose and high-dose groups(S 3.0 ml/kg+Se 2,4,8 mg/kg)with 10 mice in each group.The mice received intraperitoneal injection of Se or NS at the beginning of experiment and 10 min later received hypodermic injection of S.The times of spontaneous motor activity within 5 min were recorded 15,30,45 and 60 min after last administration,and the step-through latency,step-down latency and error times of step-down test and step-through test were recorded 1 d and 2 d after last administration.RESULTS:Compared with blank control group,the times of spontaneous motor activity in S group decreased significantly 15,30 and 45 min after medication,and step-through latency and step-down latency shortened significantly 1 d after medication,while error times increased significantly(P0.05);the times of spontaneous motor activity in drug combination low-dose,medium-dose and high-dose groups decreased significantly 15 min after medication(P0.05).Compared with S group,step-through latency and step-down latency prolonged significantly in drug combination high-dose group 1 d after medication,while the error times decreased significantly(P0.05).Other index of each group had no statistical significance(P0.05).CONCLUSIONS:S can also induce amnesia,and GABA A receptor is involved in this effect.S can decrease the times of spontaneous motor activity,but GABA A receptor isn't involved in this effect.