Separation of hydrolytically active components of cellulase from Myrothecium verrucaria by starch gel electrophoresis

Using starch gel electrophoresis according to Smithies, desalted crude cellulase from Myrothecium verrucqria was separated into at least 12 protein zones. These were tested on their activity towards p-nitrophenyl-β-D-glucoside, sodium carboxymethylcellulose and α-cellulose. They were all hydrolytically active.