Use of Ghee Residue As A Substrate For Microbial Lipase Production

ABSTRACT :- Microbial lipases are commercially important and can be produced by Solid State Fermentation (SSF) as well as by Submerged Liquid Fermentation (SLF). In this study ghee residue, which is one of the largest by-product of dairy industry was used as a substrate. The main objective of the study was to determine whether ghee residue could be used as a substrate over the conventional synthetic substrate for efficient lipase production. Two organisms were used for lipase production, one of them was a known lipase producer – Bacillus subtilis (NCIM 2063) and the other organism belonging to the Proteus spp was isolated from the soil contaminated with oil, . The production of lipase was significantly higher in SSF (ghee residue as substrate) than in SLF (tributyrin oil as substrate). The lipase yield was observed to be 41.27 U/mg by Proteus spp and35.93 U/mg by B. subtilis in SSF using gheeresidue as substrate. In SLF using Tributyrin as substrate, the lipase production by Proteus spp was 20.78 U/mg and 28.63 U/mg by B. subtilis.