miR-4458 inhibits breast cancer cell growth, migration, and invasion by targeting CPSF4

Numerous studies have implied that CPSF4 is over-expressed in a large percentage of human lung cancers and identified as a potential oncogene of human lung tumor. Down-regulation of CPSF4 inhibited lung adenocarcinoma cells proliferation and promoted lung adenocarcinoma cells apoptosis. Our previous study also suggested that CPSF4 overexpression was observed in breast cancer, and closely associated with poor prognosis. This study is to identify valuable microRNAs (miRNAs), which target CPSF4 to modulate BC cells proliferation. We found that miR-4458 was notably reduced in breast cancer tissues and cells. By using miR-4458 mimics, cells proliferation, migration, and invasion were suppressed by miR-4458 overexpression, while being enhanced by the reduction of miR-4458. Moreover, Bioinformatics analysis suggested a putative targeting site in CPSF4 3’-UTR. Furthermore, we verified that miR-4458 directly targeted to 3’-UTR of CPSF4 by luciferase reporter assay and western blot, as well as down-regulated COX-2 ...