Increasing salinity leads to differential growth and H2O2 homeostasis in plants produced from heteromorphic seeds of the succulent halophyte Arthrocnemum indicum.

Abstract Information about responses of plants grown from heteromorphic seeds is limited and inconclusive. This is especially true of subtropical halophytes where such studies have yet to be published. Therefore, growth, water-relations, and oxidative stress mitigation of plants germinated from the heteromorphic seeds of the succulent halophyte Arthrocnemum indicum under increasing (0, 300, and 900 mM NaCl) salinity were studied. Growth of plants from only small seeds was stimulated in moderate (300 mM NaCl) salinity. High (900 mM NaCl) salinity inhibited the growth of plants emerged from both small and large sized seeds. Plants germinating from both seed sizes demonstrated similar patterns of osmotic adjustment and did not develop signs of oxidative damage under increasing salinity. However, the magnitude of hydrogen peroxide and antioxidant responses differed between plant types. Under moderate salinity, plants from small seeds showed constitutive activities of most antioxidant enzymes (except superoxide dismutase) and levels of non-enzymatic antioxidants (except ascorbate). Conversely, a decline in activities of most antioxidant enzymes and levels of most non-enzymatic antioxidants occurred in plants from large seeds. While under high salinity, increased ascorbate peroxidase, glutathione, and polyphenol levels, along with unaffected ascorbate and superoxide dismutase levels, occurred in plants from small seeds. In plants from large seeds, there were increased ascorbate and polyphenol levels, but changes to the ascorbate peroxidase levels were not observed. These results thus indicate differential growth and hydrogen peroxide homeostasis in A. indicum plants emerged from heteromorphic seeds.