Analysis of expression sequence tags from Nicotiana sylvestris
2003
Partially normalized complementary DNA (cDNA) libraries were generated from root and leaf tissues of a diploid tobacco species, Nicotiana sylvestris. Single-pass sequences were obtained from a total of 13, 019 cDNA clones and were assembled into 6, 513 non-redundant cDNA groups. We estimate that these cDNA sequences represent approximately 3, 500 unique tobacco genes. The EST database described here will become a valuable resource for future research using Nicotiana species.(Communicated by Yasuyuki YAMADA, M. J. A., June 10, 2003)
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