Comparison of Two Different Methods for the Purification of Polymerase Chain Reaction (PCR) Products Used in Direct Sequencing, in an Applied Biosystems 373A DNA Sequencing System

This studywascarriedout to comparethe efficiency of two differentpurifica- tion methodsusedfor removingexcessprimersand short oligonucleotides fromPCRpro- ducts. Our aim was to sequencePCR product directly. The two different purification methodsweremicro filter spinunit system(Millipore) and QuickSpinColumn (Boehringer Mannheim Biochemica). The resultsindicatedthat the purification methodusingmicrofilter spinunit systemwas betterand yieldedbetter suquencing patterncomparedto quickSpin Column.