MICROBIOLOGIC EXAMINATION OF AIR, WATER AND FECES OF WORKERS FROM HOSPITAL KITCHENS HASTANE MUTFAKLARINDA HAVA, SU ve ÇALIŞANLARIN DIŞKILARININ MİKROBİYOLOJİK İNCELENMESİ

Objective: Food production in hygienic conditions is important for a healthy diet at all levels of food production. We aimed to perform some microbiologic analysis of air and water samples taken from the kitchens of Istanbul University, Istanbul Faculty of Medicine, and perform parasitologic analysis of feces from the kitchen personnel. Material and Method: In this descriptive study, air and water analyses were conducted between January 24 th , and March 25 th , 2011. Air samples were obtained using AES air sampler (AESAP1075/1078), 100 m 3 of air per minute was taken by impact method. Sample collection, and evaluation was made according to the ISO 14644 and ISO 14698. The prepared media for bacteria was incubated at 37° C for 2 days. The media prepared for molds was incubated at room temperature for at least five days. To obtain mold Sabouraud dextrose agar (SDA) and for total bacteria Standard Plate Count Agar (APHA) was used. Bacteria and mold colony counts were performed, and results were determined as CFU/m 3 . Water samples were taken to the (TS) 266 and were filtered using the Sartorius Stedim Membrane Filtration Mechanism. After filter papers incubated in Fluorocult LMX-Buyyon media at 37 0 C, colonies were counted in Chromocult Coliform Agar (Merk). Feces samples were taken four times between March, 2010, and March, 2011, and these samples were checked using the intensive method with formol-ether. Results: Air samples were taken from 45 areas, none of them were more than 99 CFU/m 3 for the total bacteria. In the 11 areas measured in the kitchens, in 7, total number of mold were more than 500 CFU/m 3 and in the meal service kitchens of the clinics 34 areas were measured, 7 was also more than 500 CFU/m 3 . No coliform bacterial growth was detected in any of the water samples. In parasitologic research, one staff was positive for Blastocystis hominis and one for Entamoeba histolytica/Entamoeba dispar. Conclusion: To avoid the proliferation of colonizing species of mold, precautions were taken against high temperature and humidity. Regular hygiene practices and suitable air circulation for these areas were applied.