Visualization of Mycotoxins in Living Cells UsingConformation-Resolved Aptamer Nanoprobes
2020
Mycotoxins
are the most toxic component and pose a major threat
to human health. Monitoring mycotoxins in living cells is essential
for their toxicology research; however, tools for the live-cell imaging
of mycotoxins remain unexplored. Herein, we proposed a conformation-resolved
aptamer nanoprobe (cresAptamer), allowing us to image mycotoxins in
living cells. cresAptamer endows a dual recognition mechanism: binding-induced
detachment from nanocarriers and response of intrinsic G-quadruplex
of aptamers. The dual recognition mechanism allows us to resolve conformations
of aptamer probes corresponding to nonspecific binding and target
binding, thus sufficiently supporting the specific identification
of target molecules in a complex matrix. cresAptamer significantly
improved the signal-to-background ratio for detecting ochratoxin A
(OTA), thus allowing us to sensitively and robustly image ochratoxin
A in living cells. cresAptamer would be a facile tool for dynamically
investigating the toxicology of mycotoxins at the single-cell level.
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