Synthesis of tritium-labeled 12-({sup 3}H)HPETE and 12-({sup 3}H)HPETE using 12-lipoxygenase

12-({sup 3}H)HPETE was synthesized by oxidation of ({sup 3}H)arachiodonic acid with a preparation of 12-lipoxygenase isolated and partially purified from human thrombocytes. Inhibition of peroxidase activity of the 12-lipoxygenase with indomethacin allowed us to prepare 12-({sup 3}H)PETE. The yields of 12-({sup 3}H)HPETE and 12-({sup 3}H)HETE were as high as 30-40 and 40-50% respectively. The molar radioactivity of these preparations was 140-142 Ci mmol{sup {minus}1}.