鰓黒症状を呈するクルマエビの鰓から分離された Fusarium moniliforme

Fusarium moniliforme was isolated from gill lesions of kuruma prawn Penaeus japonicus, with black gill disease at a private farm in Okinawa Prefecture in 1989. The colonies of the fungus cultured on upper surface of potato dextrose agar were floccose, creamy white, undersurface a lavender to violet, but did not grow on mycobiotic agar containing cycloheximide. The present report describes the first case of F. moniliforme infection in crustacea. An experimental infection using kuruma prawn was made by intramuscular injection with the conidia of F. moniliforme NJM 8995. For comparison, Fusarium solani NJM 8996 isolated also from a kuruma prawn with black gill disease in Okinawa Prefecture in 1989, was used as a reference. The clinical signs and path-ological findings of the disease caused by the two species of the fungi were similar. Identification of the fungi isolated from the lesions was based principally upon the cultural characteristics. The use of a media lacking cycloheximide is recommended for the isolation of F. moniliforme.