A Fully Functional, Packaged Active Microscaffold System with Fluid Perfusion and Electrical Stimulation/Recording Functionalities for 3-D Neuronal Culture Studies

This paper and presentation, for the first time, demonstrates a fully functional, packaged active microscaffold system with fluid perfusion and electrical stimulation/recording functionalities for 3-D neuronal culture studies. The fluid perfusion capabilities of the active microscaffold serve as an artificial circulatory system to enable 3-D growth and proliferation of re-aggregate neuronal cultures. Increased cell survival on microscaffolds with nutrient perfusion at 14 and 21 days in vitro (DIV) is presented. Additionally, initial characterization of the electrical stimulation/recording functionality of the microscaffold system is presented.