Prevention by antioxidants of the hemolysis of erythrocytes of cattle, pigs and humans treated with t-butyl hydroperoxide
1986
Abstract 1. Urate, 3-ribosylurate, ascorbate, glutathione and plasma protected bovine, porcine and human erythrocytes from hemolysis caused by t-butyl hydroperoxide (t-BHP). 2. Urate partially protected porcine erythrocytes from hemolysis by t-BHP when it was added 15 min after the addition of the t-BHP, but it did not protect when added 30 min after the t-BHP. 3. Glutathione and ascorbate protected oxyhemoglobin from oxidation to methemoglobin by t-BHP; 3-ribosylurate gave only slight protection. 4. Urate stimulated the formation of methemoglobin from oxyhemoglobin during treatment with t-BHP.
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