Introduction of active enzymes into intact Escherichia coli cells by means of liposomes. Phenotypic suppression of uvr A and pol A mutants.
1981
Genetically deficient cells were supplied with the missing enzymes, purified from an independent source. The introduction of exogenous enzymes into the cells was effected by two independent methods: plasmolysis and liposome transformation. The latter procedure yielded a homogenous cell population which had been rescued from the defect even if the molecular weight of the enzyme amounted to 70 KD (Kilodaltons).
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