Simple UV Spectrophotometric Estimation of Methocarbamol by Co –Solubilization Technique

Pharmaceutical analysis occupied a pivotal role in determination of drugs in formulation and its combinations. The complexity of problems in existing methods in terms of achieving the selectivity, speed, cost, simplicity, sensitivity, precision and accuracy has been replaced by new methods of analysis. The present work attempts to minimize the time consumption and cost by simple spectrophotometric method by co-solubilization technique based on the use of acetone and 0.1N sodium hydroxide solution used in the ratio of 1:9 as a solvent system. Here acetone acts as a co solvent. The drug has an absorption maximum at 267 nm and obeys Beer-Lambert's law in the concentration range of 5–25 μg/ml with correlation coefficient value of 0.999. The apparent molar absorptivity is 9.215×10 3 L mol-1cm-1. The slope and intercept of the regression equation are 3.3 ×10-2 and 5.4x10-2 respectively. The mean recovery obtained for Methocarbamol was found to be 100.56%. The optimum experimental parameters for the method have been studied. The validity of the elucidated method was assessed according to International Conference on Harmonization guidelines. Statistical analysis of the results has been carried out revealing high accuracy and good precision. The proposed method was successfully applied to the determination of methocarbamol in bulk and pharmaceutical dosage forms.