Revising the range of Rocky Mountain tailed frog, Ascaphus montanus, in British Columbia, Canada, using environmental DNA methods

2020 
We thank L. Anderson, T. Antifeau, and P. Holmes (BC Ministry of Forests, Lands & Natural Resource Operations; BC MFLNRO), A Weber‐Roy (BC Parks), M. Hounjet (Columbia Basin Trust) and C. Conroy (Ktunaxa Nation Council) for their support. We thank E. Adams, C. Browne, C. Conroy, C. Copage, T. Davis, S. Dulc, H. Flagg, E. Vincer, and A. Weber‐Roy for field and filtering assistance; K. Cochrell, M. Hii, G. Ikonomou, Dr. N. Veldhoen, and B. Walle for technical expertize; and S. Bowie for editorial review of the manuscript. Funding was provided by Innovate BC, BC MFLNRO Land Based Investment program, Columbia Basin Trust Environmental Initiatives program and BC Parks Licence Plate program. The funders were not involved in the design, interpretation or the writing of this study. A patent is pending on some of the concepts presented in the manuscript (US provisional application number 62/406,802). The patent is owned by the University of Victoria who will handle commercial and academic licensing agreements. JH was an employee of Hemmera Envirochem Inc. and is currently with J Hobbs Ecological Consulting Ltd. Ian T. Adams was employed by VAST Resource Solutions and is currently with Larix Ecological Consulting. JH and ITA were involved with all aspects of the study with the exception of running the qPCR tests used in the present study. AM and HA are employees of Bureau Veritas Laboratories and they participated in the inter‐laboratory comparative analysis of eDNA test performance.
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