An initial study of apoptosis in colorectal cancer patients and its prognostic significance

Objective The apoptosis of colorectal cancer (CRC) has been received much interest recently because of its positive rate was quite different and its influence on the prognosis of CRC was not well known.Methods Immunohistochemical MaxVision TM fast method was used to detect the expressions of Bid protein in the cell of 18 cases of normal tissues,21cases of colon adenomas and 83 cases of colon carcinoma.In 83 cases of colon carcinoma,Duckes’ stage A was 22 cases,Dukes’ stage B 20 cases,Duckes’ stage C 20 cases,and Duckes’ stage D 21 cases respectively.The semiquantitative scores method was used to observe the expression level.Results The positive rates of Bid protein in colon adenomas were 95.2% and its expressions were higher than those in normal tissues (72.2%) and CRC (72.3 %). In the CRC group the expression of Bid in Dukes' stage D(100.0%)was significantly higher than that in Dukes'stage A(40.9%) and B (55.0%),besides,28.6% of Dukes' stage D were found to be with high expression.Conclusion The positive rate and level of pro_apoptosis protein Bid in advanced CRC patients are high.The clarification of its mechanism would be of prime importance to the future biotherapy of CRC and its prognosis.