[Synchrotron radiation-based FTIR microspectroscopy study of 6-hydroxydopamine induced Parkinson's disease cell model].

SH-SY5Y cell line treated with 6-hydroxydopamine(6-OHDA) is a classical Parkinson's disease(PD).In the present study,synchrotron-based Fourier transform infrared(FTIR) microspectroscopy was used to analyze the biochemical composition of SH-SY5Y cell line treated with 6-OHDA.The detailed spectral analyses show the significant changes in cellular compositions such as lipids,proteins and nucleic acids in SH-SY5Y cells treated with 6-OHDA compared to control SH-SY5Y cells.As a result,the unsaturation levels of phospholipids decrease in SH-SY5Y cells treated with 6-OHDA compared to control cells,the analysis of protein secondary structure shows the significantly higher ratio of β-sheet in PD cells compared to that of control cells,and the content of nuclear acid is highly decreased compared to that of control cells,suggesting that 6-OHDA induces the serious oxidative damage in SH-SY5Y cells.These findings suggest that SR-FTIR is an effective and precise technical tool to probe the biochemical changes of cells and then evaluate the pathological damage in cells.