13C- and 15N-Isotopic Labeling of Proteins

Originally published in: Molecular Biology in Medicinal Chemistry. Edited by Theodor Dingermann, Dieter Steinhilber and Gerd Folkers. Copyright © 2004 Wiley-VCH Verlag GmbH & Co. KGaA Weinheim. Print ISBN: 3-527-30431-8 The sections in this article are Introduction Expression Systems for the In Vivo Incorporation of 13C and 15N Labels into Proteins Protein Production and 13C and 15N-labeling in E. coli Expression Systems 13C- and 15N-labeling of Proteins in P. pastoris 13C- and 15N-labeling of Proteins by Expression in Chinese Hamster Ovary (CHO) Cells 13C- and 15N-labeling of Proteins in Other Organisms Strategies for the Production of Selectively 13C- and 15N-labeled Proteins Selective Labeling of Amino Acids Specific Isotope Labeling with 13C Segmental Isotope Labeling Cell-free Isotope Labeling Components of Cell-free Expression Systems Cell-free Expression Techniques Specific Applications of the Cell-free Labeling Technique Keywords: molecular biology; medicinal chemistry; analysis; 13C- and 15N-isotopic labeling of proteins; expression systems; in vivo incorporation of 13C and 15N labels into proteins; cell-free isotope labeling; cell-free expression techniques