Application of UPLC- ESI-q-TOF analysis for screening of the carcinogen-modified DNA-adducts in the circulation DNA of patients with lung cancer

In this paper we have presented the development of a new approach to screening a carcinogen associated DNA adducts. As starting-material circulating DNA from blood of patients with a diagnosis of lung cancer was used. To obtain DNA adducts DNA of 100 patients and 100 healthy volunteers was hydrolyzed with 0.1 M hydrochloric acid under heating and then neutralized. The LC-MS analysis was performed on a UPLC- ESI-q-TOF mass spectrometry system with using C-18 column. Mass spectra were recorded in positive mode with 50–1000 m/z range. Simultaneously 7 DNA adducts were identified by using the approach described in this article.