Low-temperature activity type subtilisin

The present invention is, under certain circumstances, which is selected from 0 to 30 ° C., and to provide a mutant subtilisin which can exhibit excellent enzyme activity compared to the wild-type enzyme (subtilisin). Such problem is the wild-type subtilisin having the amino acid sequence represented in SEQ ID NO: 1 (wild-type enzyme), by mutant subtilisin which the first 205 amino acids counted from the N-terminal has at least mutation, is solved. Furthermore, having a first 107-th and / or the 131 amino acid has been modified variant subtilisin excellent enzyme activity.