Detection of Caprine-specific Nucleic Acid Sequences in Goat Milk Using Polymerase Chain Reaction

Conflict of interest: none declared. Introduction This study was carried out to evaluate PCR-based method for detection of DNA in goat milk. It utilized primers targeting the mitochondrial cytochrome –b (mtcyt-b) gene, which was used as a target DNA for PCR amplification. Methods For the specific identification of goat mtcyt-b gene, pair of primers (GSL1, GSR2), were used, which produced a 428 base pair (bp) PCR product from milk samples as well as from peripheral blood. Amplification products were visualized on ethidium bromide-stained agarose gels. Results and Discussion Amplification products were not detected when the PCR was applied to DNA from animal species including cattle, sheep, swine, camel, deer, horse, donkey, and human, which indicates that the 2 pairs of primers are specific for goat. Conclusion DNA can be extracted from goat milk and would be advantageous in the variety of application such as species identification in milk and milk.