HEPATOPROTECTIVE INVESTIGATIONS OF CUMINUM CYMINUM DRIED SEEDS IN NIMESULIDE INTOXICATED ALBINO RATS BY PHYTOCHEMICAL AND BIOCHEMICAL METHODS

Objective: Nimesulide, a 4-nitro-2-phenoxy methane sulphonamide is very effective non-steroidal anti-inflammatory Drug (NSAID), but at higher doses it leads to hepatotoxicity. This study was carried out on albino rats to evaluate the hepatoprotective activity of aqueous-ethanolic extract of Cuminum cyminum (Cc.E) seeds. Methods : Aqueous ethanolic extract of fresh dried cumin seed was prepared and was subjected to phytochemical analysis. For Biochemical investigations, the animals were divided into seven groups and hepatotoxicity was induced by oral administration of 100 mg/Kg Nimesulide suspension. After 15 days of treatment, the animals were dissected out and their livers were preserved for histopathological examination. Results: There was a significant increase in serum glutamic-pyruvic transaminase (SGPT), Serum glutamic oxaloacetic transaminase (SGOT), alkaline phosphatase (ALP) and serum tolal bilirubin (TB) level in intoxicated controls, which were restored towards normal in Cuminum cyminum (100, 200 and 300 mg/Kg, P.O.) treated animals. The results were compared with Silymarin (25 mg/Kg, P.O.) treated animals. Conclusion : The extract significantly (p< 0.001) reduced the serum enzyme in comparison to intoxicated control group. Furthermore, histopathological examination on the rat liver tissues supported the hepatoprotection. So, we recommend for further studies to isolate the pure component and the mechanism that displayed the hepatoprotective activity for making standard drug.