ECTOPIC OSTEOGENESIS OF BONE MARROW STROMAL CELLS INDUCED BY BONE MORPHOGENETIC PROTEIN

Objective To investigate the ectopic osteogenesis of bone marrow stromal cells (MSC) induced by bone morphogenetic protein(BMP) in vitro and in vivo , providing the experimental evidence for making an artificial bone with its own capacity of bone formation. Methods MSC were separated and cultured from bone marrow of Wistar rats, MSC were co cultured with BMP in vitro (cultured in plate and diffuse chamber). Artificial coral hydroxyapatites (CHA) with MSC and BMP were implanted into dorsal muscles of Wistar rats, their bone formation were observed by morphological examination, histochemistry and immunohistochemistry. Results Only cartilaginous matrix were produced by MSC in vitro (cultured in plate and diffuse chamber), and both cartilaginous and bone matrix production within the combined grafts were seen. The bone formation of experimental groups (CHA+BMP+MSC) was stronger than that of control A(CHA+MSC) and control B(CHA). Conclusion It may be possible to produce an artificial bone with its own capacity of bone formation by combined graft (CHA+BMP+MSC). There may be multiple factors as well as BMP inducing bone formation both in the whole body and the location of the implantation. Further research on these factors will have the significance for making the ideal artificial bone.