Effect of Triphala on a Murine Model of Isoniazid and Rifampicin Induced Model of Hepatotoxicity.

The aim of the study was to evaluate the effect of triphala on murine model of isoniazid and rifampicin induced hepatotoxicity. Thirty wistar rats of either sex, weighing 150-250 g were randomly divided into five groups of six animals each – Normal control, hepatotoxic control, Triphala 250mg group, Triphala 500mg group and standard control. Hepatotoxicity was induced in all groups except normal control, by administering isoniazid 100mg/kg body weight intraperitoneally and rifampicin 100mg/kg body weight orally, for 21 days. Triphala 250mg,Triphala 500mg and standard control groups were treated simultaneously with Triphala at doses 250mg/kg/500 mg/kg and Silymarin2.5mg/kg respectively for 21 days. The blood samples were collected by cardiac puncture under ether anesthesia, for biochemical estimation of liver enzymes, total proteins, albumin, total and direct bilirubin. Subsequently, the rats were sacrificed and the liver was dissected for histopathological evaluation. The hepatotoxic group showed significant increase in liver enzymes(P<0.001) and total and direct bilirubin (p<0.001, p=0.006 respectively), compared to normal control. Triphala 250mg, Triphala 500mg and Silymarin groups showed statistically significant decrease in liver enzymes and total bilirubin and direct bilirubin compared to hepatotoxic control (p<0.001).There was no statistically significant difference in the total protein and albumin among groups. Histopathological evaluation of liver further conferred the hepatotprotective potential of Triphala. To conclude, Triphala at doses of 250mg/kg and 500mg/kg showed hepatoprotective effect in murine model of isoniazid and rifampicin induced hepatotoxicity.