Acidovorax kalamii sp. nov., isolated from a water sample of the river Ganges
2018
A Gram-stain-negative, rod-shaped, aerobic, straw yellow, motile strain, designated KNDSW-TSA6T, belonging to the genus
Acidovorax
, was isolated from a water sample of the river Ganges, downstream of the city of Kanpur, Uttar Pradesh, India. Cells were aerobic, non-endospore-forming and motile with single polar flagella. It differed from its phylogenetically related strains by phenotypic characteristics such as hydrolysis of urea, gelatin, casein and DNA, and the catalase reaction. The major fatty acids were C16 : 1
ω7c/C16 : 1
ω6c, C16 : 0 and C18 : 1
ω7c/C18 : 1
ω6c. Phylogenetic analysis based on 16S rRNA and housekeeping genes (gyrb, recA and rpoB gene sequences), confirmed its placement within the genus
Acidovorax
as a novel species. Strain KNDSW-TSA6T showed highest 16S rRNA sequence similarity to
Acidovorax soli
BL21T (98.9 %),
Acidovorax delafieldii
ATCC 17505T (98.8 %),
Acidovorax temperans
CCUG 11779T (98.2 %),
Acidovorax caeni
R-24608T (97.9 %) and
Acidovorax radicis
N35T (97.6 %). The digital DNA–DNA hybridization and average nucleotide identity values calculated from whole genome sequences between strain KNDSW-TSA6T and the two most closely related strains
A. soli
BL21T and
A. delafieldii
ATCC 17505T were below the threshold values of 70 and 95 % respectively. Thus, the data from the polyphasic taxonomic analysis clearly indicates that strain KNDSW-TSA6T represents a novel species, for which the name Acidovorax kalamii sp. nov. is proposed. The type strain is
Acidovorax kalamii
(=MTCC 12652T=KCTC 52819T=VTCC-B-910010T).
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