Long-termPredictorofPostmenopausalBreastCancerRisk: The ORDET Prospective Study

Background: miRNAs have been implicated in the regulation of key metabolic, inflammatory, and malignant pathways; hence, they might be considered both predictors and players of cancer development. Methods: Using a case‐control study design nested in the ORDET prospective cohort study, we addressed the possibility that specific mRNAs can serve as early predictors of breast cancer incidence in postmenopausal women. We compared leukocyte miRNA profiles of 133 incident postmenopausal breast cancer cases and profiles of 133 women who remained healthy over a follow-up period of 20 years. Results: The analysis identified 20 differentially expressed miRNAs, 15 of which were downregulated. Of the 20 miRNAs, miR145-5p and miR145-3p, each derived from another arm of the respective pre-miRNA, wereconsistentlyandsignificantlydownregulatedinallthedatabasesthatwesurveyed.Forexample,analysis ofmorethan1,500patients(theUKMetabriccohort)indicatedthathighabundanceofmiR145-3pandmiR1455p wasassociated with longer, and for miR145-3p also statistically significant, survival. The experimental data attributed different rolesto the identified miRNAs: Although the 5pisoform was associated with invasion and metastasis, the other isoform seems related to cell proliferation. Conclusions:Theseobservationsandtheprospectivedesignofourstudylendsupporttothehypothesisthat downregulation of specific miRNAs constitutes an early event in cancer development. This finding might be used for breast cancer prevention. Impact: The identification of the miRNAs as long-term biomarkers of breast cancer may have an impact on breast cancer prevention and early detection. Cancer Epidemiol Biomarkers Prev; 23(11); 1–11. � 2014 AACR.