Troponin I detection kit and troponin I detection method

The invention relates to the technical field of fluorescence immune chromatography in medical immunology, and particularly relates to a troponin I detection kit and a troponin I detection method. The troponin I detection kit is provided with a test paper card, and is characterized in that the test paper card is provided with a PVC plate, a sample mat, a combination mat, a nitrocellulose membrane and a water absorption mat from bottom to top, wherein the combination mat adsorbs a troponin I monoclonal antibody marked with rare-earth fluorescent micro-spheres, the diameter of each rare-earth fluorescent micro-sphere is 60nm to 120nm, the rare-earth fluorescent micro-spheres are doped with a rare-earth lanthanide element and are stable at a ground state, and the fluorescence with the wavelength range of 540nm to 600nm can be transmitted under the effect of a 340-380nm excitation light source; the monoclonal antibody is in a (2+2) mode, namely, each of a capturing antibody and a detection antibody is a mixture of two high-quality monoclonal antibodies so as to simultaneously guarantee the specificity and affinity. The troponin I detection kit has advantages of simple operation, rapid reaction, high sensitivity, high specificity and the like.