Analysis on drug resistance of some Acinetobacter species and distribution of antimicrobial resistance genes.

Objective To investigate the drug resistance of Acinetobacter species and the distribution of antimicrobial resistance genes.Methods One hundred Acinetobacter clinical isolates,chosen by random sampling,were identified by a molecular biology approach.Non-A.baumannii strains were selected for this study.Antimicrobial susceptibility was determined with the E-test method using 12 antimicrobial agents.Sixteen antibiotic resistance genes were detected by PCR.Results Isolates of 17 non-A.baumannii strains were selected out of 100 Acinetobacter clinical isolates in this study.Seven isolates were multi-drug resistant(41.2%).All isolates were resistant to Cefotaxime.Resistance to Chloramphenicol,Ampicillin,Piperacillin,and Aztreonam was substantial,with sensitivity levels of 5.9%,11.8%,11.8%,11.8%,respectively.All isolates were sensitive to Polymyxin B.A total of 6 antibiotic resistance genes(ampC,blaTEM,blaPER-1,blaOXA-23-like,blaOXA-58 and Int1)was detected.Conclusion Extended-spectrum β-lactamase genes and blaOXA-23-like genes were the major mechanisms of resistance to β-lactam antimicrobial agents and imipenem,respectively.This study demonstrated the emergence of multi-drug resistance in the wider context of the Acinetobacter genus and reiterated the importance of surveillance of Acinetobacter resistance.