Identification, cloning and phylogenetic analysis of a y-type HMW-GS in Triticum tauschii.

Triticum tauschii(2n=2x=14,DD),as the D genome donor of common wheat,contains a wide range of high molecular weight glutenin subunit(HMW-GS) alleles,and has more genetic variation than the common wheat genome.We used sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) to analyze the HMW-GS of Aegilops tauschii,and one new y-type subunit,designated Dy8.1t was found.Through AS-PCR amplification and DNA sequencing,Dy8.1t was cloned,with a typical sequence of HMW-GS.Compared with the known subunits Dy10t,Dy10.4t,Dy10.5t and Dy12t,Dy8.1t had an amino acid difference(R-G) in the N-terminal sequence.Based on multiple sequence alignment and phylogenetic relationship analysis,Dy8.1t as a new member of HMW glutenin y-type subunits were confirmed.